CASP3F

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CASP3F

Sigma

 

Caspase 3 Assay Kit, Fluorimetric

Description

Unit DefinitionOne unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.
General descriptionThe Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Properties

usage sufficient for 1,000 multiwell tests
  sufficient for 100 standard tests (2 ml)
shipped indry ice
storage temp.−20°C

Safety

Hazard CodesXi
Risk Statements36/37/38
Safety Statements26

Components

Standard componentAc-DEVD-AMC substrate 2.5 mg A1086
 Ac-DEVD-CHO inhibitor 0.5 mg A0835
 7-Amino-4-methylcoumarin standard 1 mg A9891
Kit component only10X Assay buffer 20 mL
 Caspase 3 positive control 5 μg
 5X Lysis buffer 5 mL
 Water (17-18 megaOhm) 125 ml

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